Anti-HA Tag (Biotin) Antibody
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Catalog number
A1282-50
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Price
Please ask
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Size
50 µg
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Antibody Target
HA
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Target Alternative Name
Hemagglutinin, HA
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Antibody Type
Monoclonal
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Clone
5E11D8
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Immunogen Sequence
A synthetic peptide YPYDVPDYA coupled to KLH
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Accession
D5XIF4
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In PBS, pH 7.4, 1% BSA and 0.02% sodium azide
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Purification
Affinity purified
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Application
ELISA, WB
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Positive Control
WB: HA tagged fusion protein
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Application And Usages
ELISA: 0.05- 0.2 µg/ml, Western blot: 0.5-1 µg/ml
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Description
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
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Storage Condition
-20 °C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Verified
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Immunocytochemistry Verified
Not Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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French translation
anticorps
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Gene target
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Short name
Anti-HA Tag ( ) Antibody
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Tags
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Isotype
MouseIgG1 ĸ
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Label
tag
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Alternative name
Antibody toHA detection labelled (biotinilated) (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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