HA tag antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Monoclonal Antibodies
Area of research

Miscellaneous
Type of Immunogen

HA tag antibodies were raised in Mouse using synthetic peptide (YPYDVPDYA) derived from the human influenza hemaglutinin protein ..
Raised in

Mouse
Isotypes

IgG
Clone

2C16
Conjugate Tag

Biotin
Method of Purification

HA tag antibody was purified by affinity chromatography
Concentration

200 ug/ml
Form Buffer

Provided in PBS buffer, pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal
Storage

Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

IF; IP; WB
Usage Recommendations

IP: 2-10 ug/ml, WB: 2-10 ug/ml
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data