Anti-BrdU Antibody

• Catalog number
  A1284-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  BrdU
• Target Alternative Name
  BrdU, BUdR, BrdUrd
• Antibody Type
  Monoclonal
• Clone
  3E2D3
• Immunogen Sequence
  BrdU conjugated to KLH
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  In PBS, pH 7.4 containing 0.02% sodium azide
• Purification
  Affinity purified
• Application
  ELISA, FC, ICC/IF
• Positive Control
  FC: Hela cells, ICC/IF: Hela cells
• Application And Usages
  ELISA: 0.015- 0.05 µg/ml, Flow cytometry: 1-3 µg for 1 x 106 cells, ICC/IF: 1-3 µg/ml
• Description
  Bromodeoxyuridine (5-Bromo-2-Deoxyuridine, BrdU) is an analogue of thymidine that can be incorporated into newly synthesized DNA at the S phase of the cell cycle, thus BrdU is a common reagent used for both cell proliferation assays and for the detection of apoptotic cells. The amount of BrdU incorporated into the DNA is dependent on time exposed to BrdU and the rate of cell division. Detection of incorporated BrdU is useful to determine cell cycle kinetics, assessing cell proliferation in the presence of growth factors or cytotoxic drugs and demonstrating sister chromatid exchange. BrdU monoclonal antibody, is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with BrdU conjugated to KLH.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Not Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Verified
• ChIP Verified
  Not Verified
• Dot Blot Verified
  Not Verified
• Flow Cytometry Verified
  Verified
• Data Sheet Link
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  BrdU  
• Gene symbol
  FRA9C, FRA12C, FRA6D
• Short name
  Anti-BrdU Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  MouseIgG1 ĸ
• Alternative name
  Antibody toBrdU (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:3937
• Gene
  FRA9C
• Long gene name
  fragile site, BrdU type, common, fra(9)(p21)
• Locus
  9p21
• Discovery year
  2001-06-22

Gene info

• Identity
  HGNC:3847
• Gene
  FRA12C
• Long gene name
  fragile site, BrdU type, rare, fra(12)(q24.2)
• Locus
  12q24.2
• Discovery year
  2001-06-22

Gene info

• Identity
  HGNC:3915
• Gene
  FRA6D
• Long gene name
  fragile site, BrdU type, common, fra(6)(q13)
• Locus
  6q13
• Discovery year
  2001-06-22

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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