Sheep anti BrdU (bromodeoxyuridine)
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Catalog number
A205P
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Price
Please ask
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Size
250 μg
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Category
Primary Antibodies
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Long description
Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.
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Antibody come from
Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Sheep anti BrdU (bromodeoxyuridine) Antibody
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Antibody is raised in
Sheep
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Antibody s reacts with
N/A
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Immunoprecipitation, Immunohistochemistry
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Antibody s suited for
Applications: Western blotting: 25 -100 µg/ml; Immunoprecipitation: 25-100 µg/ml; Immunohistochemistry: 25-100 µg/ml The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 ug/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100µg/ml. _x000B_Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody.
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Storage
-20ºC
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Relevant references
1. Nakamura, S., et al. Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. Oncology 1991, 48, 285-289_x000B__x000B_2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. Biol. 1998, 80, 255-266_x000B__x000B_3. Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6)
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Gene target
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Short name
anti BrdU (bromodeoxyuridine)
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Technique
anti, antibody to
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Host
Sheep
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Isotype
not specified
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Label
unlabeled
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
ovine antibody to Bromodeoxyuridine (bromodeoxyuridine)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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