Acid Sphingomyelinase Assay Kit II (Colorimetric)
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Catalog number
K192-100
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Price
Please ask
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Size
100 assays
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Description
Complete kit that allows the measurement of Acid Sphingomyelinase in biological samples
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Detection Method
Colorimetric (OD= 570 nm)
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Species Reactivity
All
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Applications
Measurement of ASMase activity in various tissues/cell extracts
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Sample Type
Cell lysate,Serum,Tissue lysate
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Features Benefits
Complete set of reagents for the detection of Acid SMase in biological samples,Simple, specific (detect acid SMase activity only)
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Storage Conditions
-80°C
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Shipping Conditions
Dry Ice
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Shelf life
12 months
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Background
Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyelinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
Acid Sphingomyelinase Assay Kit II (Colorimetric)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
Acid Sphingomyelinase test reagent II (colorimetry)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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