Src (5B8) Monoclonal Antibody

• Catalog number
  bsm-50259M
• Price
  Please ask
• Size
  0.1ml

• Long name
  Src (5B8) Monoclonal Antibody
• Also known as
  Src (5B8) Monoclonal Antibody
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  Src
• Specificity
  This is a highly specific antibody against Src.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Monoclonal
• Clone number
  5B8
• Concentration
  4ug/ul
• Source
  Recombinant human protein purified from E.coli (His-src)
• Gene ID number
  6714
• Swiss Prot
  P12931
• Tested Applications
  WB
• Recommended dilutions
  WB (4 ug/ml)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  The members of the Src-family kinases are Src, Lyn, Fyn, Yes, Hck, Lck, Fgr, Blk, and Yrk. Each of these have a common structure consisting of an unique domain at the N-terminal, followed by SH3, SH2 and tyrosine kinase domains. In immume cells, the Src-family kinases play roles as critical regulators of a large number of intracellular signaling pathways, including integrin signaling pathway. Integrins are major cellular receptor that mediate cell to cell and cell to substratum interactions. Src is expressed ubiquitously, however the expression level is higher in brain, osteoclasts, and platelets. Src plays a role in cell adhesion, cell morphology and motility, and bone resorption. Src contains a 14-carbon myristoyl group attached to an SH4 domain, a unique domain, an SH3 domain, an SH2 domain, an SH2-kinase linker, a protein–tyrosine kinase domain (the SH1 domain), and a C-terminal regulatory segment. One of the two most important regulatory phosphorylation sites in Src is Tyr527. Under basal conditions in vivo, 90–95% of Src is phosphorylated at Tyr527, and phosphotyrosine 527 binds intramolecularly with the Src SH2 domain. Tyrosine 416 is present in the activation loop and its phosphorylation promotes kinase activity.
• Purification method
  Purified by Protein G.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  ASV; SRC1; THC6; c-SRC; p60-Src; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; SRC
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  Src   5B8   Monoclonal  
• Short name
  Anti-Src
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  Immunoglobulin G (IgG)1 κ
• Alternative name
  Src (5B8) Monoclonal Antibody
• Alternative technique
  antibodies
• Alternative to gene target
  v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian), ASV and c-SRC and p60-Src and SRC1, SRC and IDBG-74081 and ENSG00000197122 and 6714, transferase activity, nuclei, Src and IDBG-211273 and ENSMUSG00000027646 and 20779, BT.61976 and IDBG-642561 and ENSBTAG00000008938 and 535742

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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