SMURF2 Antibody
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Catalog numberbs-4056R
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PricePlease ask
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Size0.1ml
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Long nameSMURF2 Primary Polyclonal Antibody
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Also known asSMURF2 PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenSMURF2
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SpecificityThis is a highly specific antibody against SMURF2.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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Immunogen range615-665/748
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Subcellular locationNucleus, Intracellular
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SMURF2
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Gene ID number64750
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Swiss ProtQ9HAU4
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesE3 ubiquitin-protein ligase SMURF2; hSMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSMURF2
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Short nameAnti-SMURF2
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameSMURF2 Polyclonal Antibody
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Alternative techniqueantibodies
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Alternative to gene targetSMAD specific E3 ubiquitin protein ligase 2, SMURF2 and IDBG-64876 and ENSG00000108854 and 64750, SMAD binding, nuclei, Smurf2 and IDBG-213588 and ENSMUSG00000018363 and 66313, SMURF2 and IDBG-641903 and ENSBTAG00000019853 and 540826
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Gene info
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Identity
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Gene
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Long gene nameSMAD specific E3 ubiquitin protein ligase 2
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GenBank acession
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Locus
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Discovery year2004-07-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HECT domain containing
- C2 domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data