PIWIL2 (11C11) Monoclonal Antibody
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Catalog number
bsm-50298M
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Price
Please ask
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Size
0.1ml
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Long name
PIWIL2 (11C11) Monoclonal Antibody
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Also known as
PIWIL2 (11C11) Monoclonal Antibody
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
PIWIL2
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Specificity
This is a highly specific antibody against PIWIL2.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Monoclonal
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Clone number
11C11
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Concentration
1ug per 1ul
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Source
Recombinant human protein purified from E.coli (GST-mili-1)
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Gene ID number
55124
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Swiss Prot
Q8TC59
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Tested Applications
WB, IHC-P, IF(IHC-P), IP
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Recommended dilutions
WB (0.5 ug/mL) IP (5.0 ug)
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Cross reactivity
Mouse
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigen
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (By similarity). Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed:16377660).
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Purification method
Purified by Protein G.
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym names
CT80; HILI; mili; PIWIL1L; Piwi-like protein 2; Cancer/testis antigen 80; PIWIL2
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Gene symbol
PIWIL2-DT, PIWIL2
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Short name
Anti-PIWIL2
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Technique
Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Host
Mouse (Mus musculus)
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Isotype
Immunoglobulin G (IgG)1 κ
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Alternative name
PIWIL2 (11C11) Monoclonal Antibody
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Alternative technique
antibodies
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Alternative to gene target
piwi-like 2 (Drosophila), PIWIL2 and IDBG-10698 and ENSG00000197181 and 55124, piRNA binding, Cytoplasm, Piwil2 and IDBG-178442 and ENSMUSG00000033644 and 57746, PIWIL2 and IDBG-634785 and ENSBTAG00000015835 and 537068
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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