MSH2 Antibody
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Catalog numberbs-0758R
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PricePlease ask
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Size0.1ml
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Long nameMSH2 Primary Polyclonal Antibody
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Also known asMSH2 PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenMSH2
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SpecificityThis is a highly specific antibody against MSH2.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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Immunogen range80-130/935
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Subcellular locationNucleus
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MSH-2
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Gene ID number4436
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Swiss ProtP43246
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenComponent of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesFCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMSH2-OT1, MSH2
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Short nameAnti-MSH2
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameMSH2 Polyclonal Antibody
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Alternative techniqueantibodies
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Alternative to gene targetmutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli), COCA1 and FCC1 and HNPCC and HNPCC1 and LCFS2, MSH2 and IDBG-50900 and ENSG00000095002 and 4436, ADP binding, nuclei, Msh2 and IDBG-201483 and ENSMUSG00000024151 and 17685, MSH2 and IDBG-633627 and ENSBTAG00000002742 and 533115
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Gene info
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Identity
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Gene
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Long gene nameMSH2 overlapping transcript 1
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Locus
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Discovery year2019-08-15
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Entrez gene record
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RefSeq identity
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Classification
- Overlapping transcripts
Gene info
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Identity
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Gene
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Long gene namemutS homolog 2
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Synonyms gene
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Synonyms gene name
- mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
- mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1993-07-28
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Entrez gene record
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Pubmed identfication
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Classification
- MutS homologs
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data