MLH1 Antibody
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Catalog numberbs-0836R
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PricePlease ask
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Size0.1ml
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Long nameMLH1 Primary Polyclonal Antibody
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Also known asMLH1 PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenMLH1
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SpecificityThis is a highly specific antibody against MLH1.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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Immunogen range520-570/756
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Subcellular locationNucleus
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MLH1
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Gene ID number4292
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Swiss ProtP40692
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenHeterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesFCC2; COCA2; HNPCC; hMLH1; HNPCC2; DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMLH1
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Short nameAnti-MLH1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameMLH1 Polyclonal Antibody
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Alternative techniqueantibodies
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Alternative to gene targetmutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), COCA2 and FCC2 and hMLH1 and HNPCC and HNPCC2, MLH1 and IDBG-24600 and ENSG00000076242 and 4292, structure-specific DNA binding, nuclei, Mlh1 and IDBG-202755 and ENSMUSG00000032498 and 17350, MLH1 and IDBG-644253 and ENSBTAG00000016758 and 533652
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Gene info
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Identity
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Gene
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Long gene namemutL homolog 1
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Synonyms gene
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Synonyms gene name
- mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2)
- mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-11-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MutL homologs
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data