IRAK4(Thr345) Antibody , Cy7 Conjugated
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Catalog numberbs-10208R-Cy7
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Price:
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Size0.1ml
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Long nameIRAK4(Thr345) Polyclonal Antibody , Cy7 Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationCy7
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenIRAK4(Thr345)
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SpecificityThis is a highly specific antibody against IRAK4(Thr345).
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ModificationPhosphorylation
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Modification siteThr345
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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Immunogen_range330-360/460
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Subcellular locationCytoplasm
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SourceKLH conjugated Synthesised phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345
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Gene ID number51135
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Swiss ProtQ9NWZ3
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundSerine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission743nm/767nm
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SynonymsIPD1; REN64; IRAK-4; NY-REN-64; Interleukin-1 receptor-associated kinase 4; Renal carcinoma antigen NY-REN-64; IRAK4
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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ConjugatedThese antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.
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Additional conjugationCy7
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French translationanticorps
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Gene target
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Gene symbolIRAK4
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Short nameAnti-IRAK4(Thr345)
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelCy7
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Alternative nameAnti-IRAK4(Thr345) PAb Cy7
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Alternative techniqueantibodies
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Alternative to gene targetinterleukin-1 receptor-associated kinase 4, IRAK4 and IDBG-28022 and ENSG00000198001 and 51135, transferase activity, Extracellular, Irak4 and IDBG-177236 and ENSMUSG00000059883 and 266632, IRAK4 and IDBG-633310 and ENSBTAG00000021105 and 533692
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Gene info
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Identity
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Gene
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Long gene nameinterleukin 1 receptor associated kinase 4
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-09-27
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data