gentaur logogentaur logo
x

IRAK4 Antibody, FITC Conjugated

  • Catalog number
    bs-2440R-FITC
  • Product name
    IRAK4 Antibody, FITC Conjugated
  • Size
    0.1ml
  • Short name
    Anti-IRAK4
  • Long name
    IRAK4 Polyclonal Antibody, FITC Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    FITC
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    IRAK4
  • Specificity
    This is a highly specific antibody against IRAK4.
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Isotype
    Immunoglobulin G (IgG)
  • Concentration
    1ug per 1ul
  • Immunogen range
    20-55/460
  • Source
    KLH conjugated synthetic peptide derived from human IRAK4
  • Gene ID number
    51135
  • Swiss Prot
    Q9NWZ3
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    494nm/518nm
  • Synonyms
    IPD1; REN64; IRAK-4; NY-REN-64; Interleukin-1 receptor-associated kinase 4; Renal carcinoma antigen NY-REN-64; IRAK4
  • Alternative to gene target
    • [ "interleukin-1 receptor-associated kinase 4", "IRAK4 and IDBG-28022 and ENSG00000198001 and 51135", "transferase activity", "Extracellular", "Irak4 and IDBG-177236 and ENSMUSG00000059883 and 266632", "IRAK4 and IDBG-633310 and ENSBTAG00000021105 and 533692" ]
  • Gene target
    IRAK4 Conjugated
    • Gene info
    Locus Specific Databases:IRAK4base: Mutation registry for IRAK4 deficiencyLRG_75
  • Gene symbol
    IRAK4
  • isotype filter
    • Immunoglobulin G IgG
  • Label
    FITC
  • label filter
    • FITC
  • technique filter
    • Antibody
    • FITC
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
  • Alternative technique
    antibodies, fluorescine