CD8 Antibody, Biotin Conjugated
-
Catalog number
bs-0648R-Biotin
-
Price
Please ask
-
Size
0.1ml
-
-
Long name
CD8 Polyclonal Antibody, Biotin Conjugated
-
Category
Conjugated Primary Antibodies
-
Conjugation
Biotin
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
CD8
-
Specificity
This is a highly specific antibody against CD8.
-
Modification
Unmodified
-
Modification site
None
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen_range
50-90/247
-
Subcellular location
Extracellular
-
Source
KLH conjugated synthetic peptide derived from mouse CD8
-
Gene ID number
12525
-
Swiss Prot
P01731
-
Tested applications
WB, IHC-P
-
Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
-
Crossreactivity
Mouse, Rat
-
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen background
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission
N/A
-
Synonyms
Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Lyt2
-
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Additional conjugation
Biotinylated
-
Description
Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
-
French translation
anticorps
Gene info
-
Identity
-
Gene
-
Long gene name
CD8a molecule
-
Synonyms gene
-
Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
-
Locus
-
Discovery year
1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- V-set domain containing
- CD molecules
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products