c-Raf(Tyr341) Antibody, Cy3 Conjugated
-
Catalog number
bs-5650R-Cy3
-
Price
Please ask
-
Size
0.1ml
-
-
Long name
c-Raf(Tyr341) Polyclonal Antibody, Cy3 Conjugated
-
Category
Conjugated Primary Antibodies
-
Conjugation
Cy3
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
c-Raf Tyr341
-
Specificity
This is a highly specific antibody against c-Raf Tyr341.
-
Modification
Phosphorylation
-
Modification site
Tyr341
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Immunogen_range
325-375/648
-
Subcellular location
Cytoplasm, Nucleus, Mitochondrion, Cell membrane
-
Source
KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Tyr341
-
Gene ID number
5894
-
Swiss Prot
P04049
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human
-
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen background
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission
512,550nm/570,615nm
-
Synonyms
NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; RAF1; RAF
-
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-c-Raf Tyr341 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
-
Additional conjugation
cy3 conjugation kit
-
French translation
anticorps
-
Gene target
-
Short name
Anti-c-Raf Tyr341
-
Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
-
Isotype
Immunoglobulin G (IgG)
-
Label
Cy3
-
Alternative name
Anti-c-Raf Tyr341 PAb Cy3
-
Alternative technique
antibodies
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products