A Raf (Ser214) Antibody, Cy3 Conjugated
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Catalog numberbs-3005R-Cy3
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Price:
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Size0.1ml
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Long nameA Raf (Ser214) Polyclonal Antibody, Cy3 Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationCy3
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenA Raf Ser214
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SpecificityThis is a highly specific antibody against A Raf Ser214.
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ModificationPhosphorylation
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Modification siteSer214
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser214
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Gene ID number369
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundThe RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission512,550nm/570,615nm
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SynonymsA raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 murine sarcoma 3611 virus; ARAF_HUMAN.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-A Raf Ser214 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
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Additional conjugationcy3 conjugation kit
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French translationanticorps
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Gene target
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Short nameAnti-A Raf Ser214
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelCy3
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Alternative nameAnti-A Raf Ser214 PAb Cy3
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data