ATG3 (Tyr18) Antibody, HRP Conjugated
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Catalog number
bs-5204R-HRP
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Price
Please ask
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Size
0.1ml
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Long name
ATG3 (Tyr18) Polyclonal Antibody, HRP Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
HRP
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
ATG3 Tyr18
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Specificity
This is a highly specific antibody against ATG3 Tyr18.
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Modification
Phosphorylation
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Modification site
Tyr18
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
KLH conjugated synthetic phosphopeptide derived from human ATG3 around the phosphorylation site of Tyr18
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Gene ID number
64422
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Tested applications
WB, IHC-P
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
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Crossreactivity
Human, Mouse, Rat
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
N/A
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Synonyms
2610016C12Rik; Apg 3; APG3; APG3 autophagy 3 like; APG3 like; APG3-like; APG3L; Apg3p; ATG 3; ATG3; ATG3 autophagy related 3 homolog; ATG3 autophagy related 3 homolog S. cerevisiae; ATG3_HUMAN; Autophagy Apg3p/Aut1p like; Autophagy related protein 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201; OTTHUMP00000214547; OTTHUMP00000214548; PC3 96; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3.
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Additional conjugation
HRP Conjugated
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French translation
anticorps
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Gene target
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Gene symbol
ATG3
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Short name
Anti-ATG3 Tyr18
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Technique
Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
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Isotype
Immunoglobulin G (IgG)
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Label
HRP
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Alternative name
Anti-ATG3 Tyr18 PAb HRP
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Alternative technique
antibodies
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Alternative to gene target
autophagy related 3, ATG3 and IDBG-49891 and ENSG00000144848 and 64422, enzyme binding, Cytoplasm, Atg3 and IDBG-162821 and ENSMUSG00000022663 and 67841, ATG3 and IDBG-632300 and ENSBTAG00000009084 and 508571
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Gene info
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Identity
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Gene
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Long gene name
autophagy related 3
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Synonyms gene
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Synonyms gene name
- APG3 autophagy 3-like (S. cerevisiae)
- ATG3 autophagy related 3 homolog (S. cerevisiae)
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Synonyms
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Locus
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Discovery year
2004-07-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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