Acetyl-Histone H4(K12) Antibody, FITC Conjugated
-
Catalog number
bs-3746R-FITC
-
Price
Please ask
-
Size
0.1ml
-
-
Long name
Acetyl-Histone H4(K12) Antibody, FITC Conjugated
-
Category
Conjugated Primary Antibodies
-
Conjugation
FITC
-
Host Organism
Rabbit (Oryctolagus cuniculus)
-
Target Antigen
Acetyl-Histone H4K12
-
Specificity
This is a highly specific antibody against Acetyl-Histone H4K12.
-
Modification
Acetylation
-
Modification site
K12
-
Clonality
Polyclonal
-
Clone
Polyclonal antibody
-
Concentration
1ug per 1ul
-
Source
KLH conjugated synthetic acetylpeptide derived from human Histone H4 around acetylation site of Lys12
-
Gene ID number
121504
-
Tested applications
IF(IHC-P)
-
Recommended dilutions
IF(IHC-P)(1:50-200)
-
Crossreactivity
Human, Mouse, Rat
-
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
-
Antigen background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].
-
Purification method
This antibody was purified via Protein A.
-
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
-
Excitation Emission
494nm/518nm
-
Synonyms
Histone H4 Acetyl K12; Histone H4 Acetyl Lys12; Acetyl-Histone H4 Lys12; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN.
-
Description
N terminal acetylation or CH3CO as epigenetic regulation of Anti-Acetyl-Histone H4K12 by NATs.
-
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
-
Additional conjugation
Anti-FITC Antibody
-
French translation
anticorps
-
Gene target
-
Gene symbol
SECTM1, KRT12
-
Short name
Anti-Acetyl-Histone H4K12
-
Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
-
Isotype
Immunoglobulin G (IgG)
-
Label
FITC
-
Alternative name
Anti-Acetyl-Histone H4K12 PAb FITC
-
Alternative technique
antibodies, fluorescine
-
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
keratin 12
-
Synonyms gene name
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year
1997-07-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products