5-HT Antibody, FITC Conjugated
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Catalog numberbs-1126R-FITC
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PricePlease ask
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Size0.1ml
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Long name5-HT Polyclonal Antibody, FITC Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationFITC
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target Antigen5-HT
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SpecificityThis is a highly specific antibody against 5-HT.
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ModificationUnmodified
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Modification siteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated to 5-HT
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityOthers
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Crossreactive species details5-HT
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Antigen backgroundSerotonin (pronounced) (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system (CNS) and enterochromaffin cells in the gastrointestinal tract of animals including humans. Serotonin is also found in many mushrooms and plants, including fruits and vegetables.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission494nm/518nm
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SynonymsSerotonin; 5-hydroxytryptamine; 3-2-Aminoethyl-5-hydroxyindole hydrochloride; 5-HT, 5-Hydroxytryptamine hydrochloride.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolHTR2B, MIR1302-5, PIRC22, PIRC23, PIRC20, PIRC21, HTR6, IGKV3OR2-5, IGHV2OR16-5, SNORD116-5
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Short nameAnti-5-HT
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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IsotypeImmunoglobulin G (IgG)
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LabelFITC
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Alternative nameAnti-5-HT PAb FITC
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene name5-hydroxytryptamine receptor 2B
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Synonyms gene name
- 5-hydroxytryptamine (serotonin) receptor 2B
- 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled
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Synonyms
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Locus
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Discovery year1994-07-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 5-hydroxytryptamine receptors, G protein-coupled
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-5
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 22
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 23
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 20
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 21
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene name5-hydroxytryptamine receptor 6
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Synonyms gene name
- 5-hydroxytryptamine (serotonin) receptor 6
- 5-hydroxytryptamine (serotonin) receptor 6, G protein-coupled
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 5-hydroxytryptamine receptors, G protein-coupled
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 3/OR2-5 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 3/OR2-5
- immunoglobulin kappa variable 3/OR2-5 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 2/OR16-5 (non-functional)
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Synonyms gene name
- immunoglobulin heavy variable 2/OR16-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 116-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data