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Also known as
Vimentin
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Other names
vimentin; Vimentin; vimentin; vimentin; N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
VIM; VIM; CTRCT30; N/A
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Gene name synonims
VIM; VIM; CTRCT30; N/A
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Other gene names
VIM; VIM; CTRCT30; N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
RV202
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Host organism
Mouse (Mus musculus)
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Species reactivity
Canine, Chicken, Caprine, Human (Homo sapiens), Monkey, Mouse (Mus musculus), Rat (Rattus norvegicus), Swine, Zebrafish; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
RV202-FITC reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-FITC is suitable for immunocytochemistry on cell cultures and cytospins, immuno-histochemistry on frozen tissues, as well as for flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:10.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
Each vial contains 1ml FITC-conjugated vimentin monoclonal antibody in PBS containing 0,1% BSA, 0.09% sodium azide. Approximately 100 tests.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius., or in small aliquots the antibody should be stored at -20 degrees Celsius..
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Tested applications
Flow Cytometry (FC), Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
