Anti-MAP 1A/1B LC3 A/B (Polyclonal), ALEXA Fluor 594
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Catalog numberGENTObs-4309R-A594
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PricePlease ask
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Size100 microliters
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TypeConjugated Primary Antibody
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Conjugated withALEXA FLUOR® 594
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Host organismRabbit (Oryctolagus cuniculus)
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Target Protein PeptideMAP 1A/1B LC3 Alpha/Beta
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SpecificityThis antibody reacts specifically with MAP 1A/1B LC3 A/B
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ModificationNo modification has been applied to this antibody
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Modification siteNone
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ClonalityPolyclonal Antibody
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ClonePolyclonal Antibodies
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Concentration1ug per 1ul
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Subcellular locationsCytoplasm, Cell membrane
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Antigen SourceKLH conjugated synthetic peptide derived from human MAP LC3 Alpha
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Gene ID54901, 84557
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Swiss ProtQ9H492, Q9GZQ8
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ApplicationsIF(IHC-P)
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Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
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Cross reactive speciesHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species detailsNo significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background informationMicrotubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3 alpha) are subunits of both MAP1A and MAP1B. MAP-LC3M Beta, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3 Beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3∫, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3 Beta may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.
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Purification methodPurified by Protein A.
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StorageWater buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission590nm/617nm
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SynonymsMicrotubule-associated proteins 1A/B light chain 3A; Microtubule-associated proteins 1A/B light chain 3B; Microtubule-associated proteins 1Beta light chain 3A; Microtubule-associated proteins 1Beta light chain 3A; MAP1B LC3 A; MAP LC3 Beta; MAP-LC3 Beta; MAP1 light chain 3-like protein 1.MAP1LC3B.MLP3A_HUMAN
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Also known asMAP 1A/1B LC3 A/B Polyclonal Antibody
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Other nameAnti-MAP 1A/1B LC3 A/B Polyclonal
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AdvisoryAvoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbolMOAP1, MAP7, MAP1LC3A, MASP2, MASP1
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Short nameAnti-MAP 1A/1B LC3 A/B
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TechniquePolyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeImmunoglobulin G (IgG)
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LabelALEXA FLUOR 594
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Alternative nameMAP 1A/1B LC3 A/B Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative techniquepolyclonals, antibodies
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Gene info
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Identity
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Gene
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Long gene namemodulator of apoptosis 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-11-13
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Entrez gene record
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Pubmed identfication
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Classification
- Paraneoplastic Ma antigens
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule associated protein 1 light chain 3 alpha
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Synonyms
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Locus
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Discovery year2000-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Autophagy related
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameMBL associated serine protease 2
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Synonyms gene
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Synonyms gene name
- mannan-binding lectin serine protease 2
- mannan-binding lectin serine peptidase 1 pseudogene 1
- mannan-binding lectin serine protease 1 pseudogene 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-12-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sushi domain containing
- Complement system activation components
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameMBL associated serine protease 1
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Synonyms gene
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Synonyms gene name
- mannan-binding lectin serine protease 1 (C4/C2 activating component of Ra-reactive factor)
- mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor)
- mannan binding lectin serine peptidase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-07-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serine proteases
- Complement system activation components
- Sushi domain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
