Iscove’s Modified DMEM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 10L

• Catalog number
  PM-I1093/10L
• Price
  Please ask
• Size
  4X 10L

• Description
  Iscove’s Modified DMEM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 10L is used for the rapid proliferation of high density cell cultures.
• Similar product
• Shipping conditions
  -20°C or dry ice
• Storage
  + 2 / + 8 ° C 
• Shelflife
  12 months
• State
  Liquid
• Chemical description
  Water-based mixture of inorganic and organic compounds
• Appearance
  Physical state at 20 ºC: Liquid ; Appearance: Fluid ; Colour: Red
• Density at 20 ºC
  1040 kg/m³
• Relative density at 20 ºC
  1.04
• PH
  7.2 ± 0.3
• Osmolality
  276 ±10% mOsm/kg
• Endotoxin
  < 1 EU/ml
• Cell growth test
  Medium tested for the ability to support SP2/0-Ag14 cell growth.
• Composition
  NA
• Sterility
  Sterilizing filtration 
• Personal precautions
  Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
• Uses
  Used in biotechnology. For professional user/industrial user only.
• Technical data
• Data sheet
• Ordering
  To order Iscove’s Modified DMEM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 10L, please use the Cat. Nr.PM-I1093/10L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for Iscove’s Modified DMEM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM Hepes - For 10L
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  Iscove’s   Modified   DMEM   L-Glutamine   w/o   Sodium   Bicarbonate   For   10L  
• Short name
  Iscove’s Modified DMEM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 mM - For 10L
• Alternative name
  Iscove’s Modified DMEM w/ L-Glutamine w/o Sodium Bicarbonate w/ 25 millimeter Hepes - to measure 10L

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