DMEM - F12 w/ L-Glutamine w/o Sodium Bicarbonate w/ 15 mM Hepes - For 10L

• Catalog number
  PM-D1227/10L
• Price
  Please ask
• Size
  5X 10L

• Description
  DMEM - F12 w/ L-Glutamine w/o Sodium Bicarbonate w/ 15 mM Hepes - For 10L is used in the maintenance and the culturing of mammalian cells. It offers excellent performance for certain epithelial, endothelial and granulose cell types.
• Similar product
• Shipping conditions
  -20°C or dry ice
• Storage
  + 2 / + 8 ° C 
• Shelflife
  36 months
• State
  Powder
• Chemical description
  Mixture composed of inorganic substanced
• Appearance
  Physical state at 20 ºC: Solid ; Appearance: Powdery
• Density at 20 ºC
  1737 kg/m³
• Relative density at 20 ºC
  1.037
• PH
  5.8 ± 0.3
• Osmolality
  279 mOsm/kg±10%
• Endotoxin
  < 1 EU/ml
• Cell growth test
  NA
• Composition
  NA
• Sterility
  Non-sterile 
• Personal precautions
  Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
• Uses
  Used in biotechnology. For professional user/industrial user only.
• Technical data
• Data sheet
• Ordering
  To order DMEM - F12 w/ L-Glutamine w/o Sodium Bicarbonate w/ 15 mM Hepes - For 10L, please use the Cat. Nr.PM-D1227/10L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
• Tips
  Our specialists recommend you to follow carefully the pre-registered instructions for DMEM - F12 w/ L-Glutamine w/o Sodium Bicarbonate w/ 15 mM Hepes - For 10L
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  DMEM   F12   L-Glutamine   w/o   Sodium   Bicarbonate   For   10L  
• Gene symbol
  F12
• Short name
  DMEM - F12 w/ L-Glutamine w/o Sodium Bicarbonate w/ 15 mM - For 10L
• Alternative name
  DMEM - F12 w/ L-Glutamine w/o Sodium Bicarbonate w/ 15 millimeter Hepes - to measure 10L

Gene info

• Identity
  HGNC:3530
• Gene
  F12
• Long gene name
  coagulation factor XII
• Synonyms gene name
  • coagulation factor XII (Hageman factor)
• GenBank acession
  • M31315
• Locus
  5q35.3
• Discovery year
  2001-06-22
• Entrez gene record
  2161
• RefSeq identity
  • NM_000505
• VEGA ID
  OTTHUMG00000163403
• Locus Specific Databases
  • LRG_145

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