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Description
HEPES Buffer 1 M - 500ml is a buffer solution that is used in cell culture media.
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Shipping conditions
-20°C or dry ice
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Storage
+2/+8°C
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Shelflife
36 months
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State
Liquid
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Chemical description
Buffer solution
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Appearance
Physical state at 20 ºC: Liquid ; Appearance: Fluid ; Colour: Colourless
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Density at 20 ºC
1107 kg/m³
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Relative density at 20 ºC
1.107
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PH
7.3 ± 0.3
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Osmolality
> 1000 mOsm/kg
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Endotoxin
< 1 EU/ml
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Composition
238.31 g/l of HEPES in distilled water
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Sterility
Sterile
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Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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Uses
Usage biotechnologique. Uniquement pour usage professionnel.
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Ordering
To order HEPES Buffer 1 M - 500ml, please use the Cat. Nr.LM-S2030/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for HEPES Buffer 1 M - 500ml
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Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Biosera.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
