MEM w/ Hanks' Salts w/o L-Glutamine w/ 25 mM Hepes - 500ml
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Catalog number
LM-H1147/500
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Price
Please ask
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Size
15X500ml
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Description
MEM w/ Hanks' Salts w/o L-Glutamine w/ 25 mM Hepes - 500ml is used for the growth and maintenance of a wide range of mammalian cell types.
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Shipping conditions
-20°C or dry ice
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Storage
+ 2 / + 8 ° C
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Shelflife
24 months
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State
Liquid
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Chemical description
NA
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Appearance
NA
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Density at 20 ºC
NA
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Relative density at 20 ºC
NA
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PH
7.2 ± 0.3
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Osmolality
300 mOsm/kg ± 10 %
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Cell growth test
Medium tested for the ability to support L929 or MRC5 cell growth.
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Composition
NA
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Sterility
Sterile
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Personal precautions
Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask, hygiene cap, overall…)
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Uses
Usage biotechnologique. Uniquement pour usage professionnel.
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Data sheet
Contact us to request a data sheet
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Ordering
To order MEM w/ Hanks' Salts w/o L-Glutamine w/ 25 mM Hepes - 500ml, please use the Cat. Nr.LM-H1147/500 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for MEM w/ Hanks' Salts w/o L-Glutamine w/ 25 mM Hepes - 500ml
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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