Long-Taq DNA Polymerase (5U/ul), with 10X buffer (Mg2+ Plus) and PCR Enhancer
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Catalog number
A4186-2X250U
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Price
Please ask
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Size
2X250U
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Storage Temperature
Long-Taq DNA Polymerase (5U/ul), with 10X buffer (Mg2+ Plus) and PCR Enhancer should be stored at -20°C
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Shipping Conditions
Ice pack
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Retest
2 years
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Application
For research use only. Not for diagnostic procedures, drug use, or for administration to humans or animals.
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Hazardous
NA
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Ordering
To order Long-Taq DNA Polymerase (5U/ul), with 10X buffer (Mg2+ Plus) and PCR Enhancer , please use the Cat. Nr. A4186-250U and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Long-Taq DNA Polymerase (5U/ul), with 10X buffer (Mg2+ Plus) and PCR Enhancer
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Description
Buffering solutions are useful to keep the pH range sable when using this reagent of Biomatik.
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Group
PCR, polymerase chain reaction
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About
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Properties
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
Long-Taq DNA Polymerase (5U/ul), with 10X (Mg2+ Plus) PCR Enhancer
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Technique
dna, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Biomatik TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Alternative name
large-Taq Desoxyribonucleic acid polymerizing enzyme (5U/ul), including 10X solution (Mg2+ Plus) and PCR test kit Enhancer
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Alternative technique
dna-amplification
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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