Human Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit, 96 well plate
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Catalog numberEKU03716
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PricePlease ask
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Size96T
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Verified reactivityHomo sapiens (Human)
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Detection limits31.25-2000 pg/mL
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Sensitivity limit13,9 pg/mL
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Samples to be analyzedSerum, plasma, tissue homogenates and other biological fluids
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Assay duration3 hours
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Verified applicationsELISA
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Assay classSandwich
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ELISA detectionColorimetric
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Protein numberO75907
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Alternate protein numberPlease refer to SwissProt
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Gene number8694
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Gene nameDiacylglycerol-O-Acyltransferase Homolog 1
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Alternate gene nameARGP1; DGAT; ARAT; ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase; Diglyceride acyltransferase
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Estimated production time7-11 business days
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Shipping requirementsBlue ice
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Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Use for8 months
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ELISA s specificityThis assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) and analogues was observed.
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ELISA s cross reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
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Assay principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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ProtocolPlease see ELISA's datasheet, otherwise contact us
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Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
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ELISA s stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
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Research main areaEnzyme & Kinase;
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WarningsThe Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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NotesFor research use only. Not for diagnostic procedures.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
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Gene target
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Gene symbolCENPO, H4C15, POFUT1, SDR9C7, GNAO1, H3C15, H2BC19P, FABP3, OGT, AOPEP
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Short nameDiacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) ELISA Kit, 96
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TechniqueELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Hostantigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesHuman, Humans
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Alternative nameH. sapiens Diacylglycerol-O-Acyltransferase Homolog 1 (DGAT1) Enzyme-linked immunosorbent assay reagent, 96 well plate
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Alternative techniqueelisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene namecentromere protein O
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-06-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Constitutive centromere associated network
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH4 clustered histone 15
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Synonyms gene
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Synonyms gene name
- histone 2, H4b
- histone cluster 2, H4b
- histone cluster 2 H4 family member b
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-06-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H4 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein O-fucosyltransferase 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-10-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Fucosyltransferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameshort chain dehydrogenase/reductase family 9C member 7
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Synonyms gene name
- short chain dehydrogenase/reductase family 9C, member 7
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-12-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Short chain dehydrogenase/reductase superfamily
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameG protein subunit alpha o1
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Synonyms gene name
- guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
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Synonyms
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Locus
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Discovery year1988-04-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- G protein subunits alpha, group i
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH3 clustered histone 15
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Synonyms gene
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Synonyms gene name
- histone 2, H3a
- histone cluster 2, H3a
- histone cluster 2 H3 family member a
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-19
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Entrez gene record
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Pubmed identfication
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Classification
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 19, pseudogene
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Synonyms gene
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Synonyms gene name
- histone 2, H2bd
- H2B histone family, member O
- histone cluster 2, H2bd (pseudogene)
- histone cluster 2 H2B family member d (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefatty acid binding protein 3
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Synonyms gene
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Synonyms gene name
- fatty acid binding protein 11
- fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
- fatty acid binding protein 3, muscle and heart
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-08-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fatty acid binding protein family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameO-linked N-acetylglucosamine (GlcNAc) transferase
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Synonyms gene name
- O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-12-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- O-linked N-acetylglucosaminyltransferases
- NSL histone acetyltransferase complex
- Tetratricopeptide repeat domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameaminopeptidase O (putative)
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Synonyms gene
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Synonyms gene name
- chromosome 9 open reading frame 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-01-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- M1 metallopeptidases
- MicroRNA protein coding host genes
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VEGA ID
MeSH Data
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Name
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ConceptScope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data