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Available ordering format
Lyophilized
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Immunogen
recombinant CrDGAT2A, overexpressed in E.coli, missing transmembrane domains, PID 536226, also annotated as DGTT1 UniProt: A8JGY1
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 100 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
Algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB)
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Molecular weight expected аpparent
kDa
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Verified reactivity
Chlamydomonas reinhardtii
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Possible reactivity
Chlamydomonas reinhardtii
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
to be added when available
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References
Liu et al. (2016). A simple and reproducible non-radiolabeled in vitro assay for recombinant acyltransferases involved in triacylglycerol biosynthesis. J Appl Phycol (2016). doi:10.1007/s10811-016-0949-6. Wase et al. (2015). Phenotypic screening identifies Brefeldin A/Ascotoxin as an inducer of lipid storage in the algae Chlamydomonas reinhardtii. Algal Research, Volume 11, September 2015, Pages 74-84.
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Scientific context
DGAT2A (Acyl-CoA: Diacylglycerol acyltransferase) is an enzyme which exhibits transferase activity, transferring acyl groups. EC=2.7.8.2.
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Notes
None
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Protein number
536226
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TAIR number
Refer to NCBI
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