HS-Taq PCR Master Mix, 2X

Chemical grade

Molecular Biology
Storage requirements

-20 °C
Category

DNA & PCR
Hazardous number

Please see datasheet or contact us for more information and details about hazardous materials.
Chemical class

Please see datasheet
Chemical PG

No Data Available
Supplementary technical information

Contact us for more technical information
Notes

For research use only. Not for diagnostic procedures.
Hyperlink
Group

PCR, polymerase chain reaction
About

TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Properties

Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.

Gene target

HS-Taq Master
Gene symbol

TRADD, NRXN1, APPBP2, HSPB2, CLDN7, MAP3K14, BCS1L, NPRL3, HSPB1, MPPED2
Short name

HS-Taq PCR Master , 2X
Technique

PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. bioma TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
Alternative name

HS-Taq PCR test kit Master Mix, 2X
Alternative technique

dna-amplification

Identity

HGNC:622
Gene

APPBP2
Long gene name

amyloid beta precursor protein binding protein 2
Synonyms gene name
- amyloid beta precursor protein (cytoplasmic tail)-binding protein 2
- amyloid beta precursor protein (cytoplasmic tail) binding protein 2
Synonyms
- KIAA0228
- Hs.84084
- PAT1
Synonyms name
- protein interacting with APP tail 1
GenBank acession
- AF017782
Locus

17q23.2
Discovery year

1999-06-25
Entrez gene record

10513
Pubmed identfication
- 9843960
RefSeq identity
- NM_006380
VEGA ID

OTTHUMG00000180048

Identity

HGNC:1020
Gene

BCS1L
Long gene name

BCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone
Synonyms gene name
- BCS1 (yeast homolog)-like
- BCS1-like (yeast)
- BCS1-like (S. cerevisiae)
- BC1 (ubiquinol-cytochrome c reductase) synthesis-like
Synonyms
- Hs.6719
- BCS
- h-BCS
- BJS
Synonyms name
- GRACILE syndrome
- Bjornstad syndrome
GenBank acession
- AF026849
Locus

2q35
Discovery year

1998-07-03
Entrez gene record

617
Pubmed identfication
RefSeq identity
- NM_004328
Classification
- Mitochondrial respiratory chain complex assembly factors
- AAA ATPases
VEGA ID

OTTHUMG00000133114
Locus Specific Databases
- LRG_539

Identity

HGNC:14124
Gene

NPRL3
Long gene name

NPR3 like, GATOR1 complex subunit
Synonyms gene
- C16orf35
Synonyms gene name
- chromosome 16 open reading frame 35
- nitrogen permease regulator-like 3 (S. cerevisiae)
- NPR3-like, GATOR1 complex subunit
Synonyms
- CGTHBA
- RMD11
- NPR3
- MARE
- HS-40
Synonyms name
- conserved gene telomeric to alpha globin cluster
Locus

16p13.3
Discovery year

2000-12-21
Entrez gene record

8131
Pubmed identfication
- 8575760
RefSeq identity
- NM_001039476
Classification
- GATOR1 subcomplex
VEGA ID

OTTHUMG00000047792

Identity

HGNC:5246
Gene

HSPB1
Long gene name

heat shock protein family B (small) member 1
Synonyms gene name
- heat shock 27kD protein 1
- heat shock 27kDa protein 1
Synonyms
- HSP27
- HSP28
- Hs.76067
- Hsp25
- CMT2F
GenBank acession
- X54079
Locus

7q11.23
Discovery year

1991-07-09
Entrez gene record

3315
Pubmed identfication
- 2243808
- 9344682
Classification
- Small heat shock proteins
VEGA ID

OTTHUMG00000023228
Locus Specific Databases
- Inherited Peripheral Neuropathies Mutation Database
- LRG_248

Name

Nucleic Acid Amplification Techniques
Concept

Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Tree numbers
- E05.393.620
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data