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Availability
Please contact us to check the availability
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Immunogen
Human TSH
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Specificity
Human thyroid stimulating hormone (TSH), no cross-reactivity with LH, FSH and HCG
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Species reactivity
Human
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**.
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
Ig
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Antibody s clone
polyclonal
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:10 - 1:50
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Protein concentration
N/A
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Positive control
Human pituitary
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Pretreatment
Pre-treatment with proteases not required. Antigen-retrieval with HIER not established.
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Tested applications
IHC(P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art. No. AE005), for higher sensitivities VECTASTAIN Elite ABC (Art. No. PK-6101) or ABC AP (Art. No. AK-5001) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Losa M., Giovanelli M., Persani L., Mortini P., Faglia G., and Beck-Peccoz P. (1996) Criteria of cure and follow-up of central hyperthyreoidism due to thyrotropin-secreting pituitary adenomas. J. Clin. Endocrinol. Metab. 81(8): 3084-3090. 2. Yamakita N., Ikeda T., Murai T., Komaki T., Hirata T., and Miura K. (1995) Thyrotropin-producing pituitary adenoma discovered as a pituitary incidentaloma. Intern. Med. 34(11): 1055-1060.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Hormone releasing factors and releasing hormones are signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
