-
Availability
Please contact us to check the availability
-
Immunogen
Recombinant 150 aa peptide of the C-terminal human Nestin protein, conjugated to GST
-
Specificity
Human Nestin (200-220 kDa in WB)
-
Species reactivity
Human, no cross-reaction with mouse and rat
-
Product presentation
Purified IgG1 in PBS pH 7.3 with 0.1% sodium azide, BSA and gelatine
-
Product category
Primary Antibody
-
Antibody s raised in
Mouse
-
Antibody s isotype
IgG1
-
Antibody s clone
10c2
-
Product is supplied as
liquid concentrate
-
Recommended concentration for use
WB 1:100-1:1000, IHC 1:50-1:200, IP 1:50-1:100 (for 100 µg/ml cell lysate)
-
Protein concentration
100 µg/ml
-
Positive control
Brain, (SK-N-SH or SH-SY5Y cells for Western Blot)
-
Pretreatment
Formalin-fixed tissue requires heat-induced antigen retrieval. We recommend the use of citrate buffer pH 6 (Art. No. DE000) 10-20 min at 100°C, thereafter cooling for 20 min until RT is reached.
-
Tested applications
WB 1:100-1:1000, IHC 1:50-1:200, IP 1:50-1:100 (for 100 µg/ml cell lysate)
-
Recommended secondary reagent
Anti-mouse IgG:Biotin conjugate (code no. ZU102) in combination with Streptavidin-HRPO (code no. ZU054) and a suitable substrate e.g. DAB (Ref. DA002) or AEC (Ref AE002).
-
Storage method
Store at 2-8°C
-
UniProt number
P48681 (NEST_HUMAN)
-
Litterature
1. Hockfield,S. and McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system. J Neurosci. 5: 3310-3328. 2. V.A. Förenes R. Holm, O. Myklebost, U. Lendahl, and Öystein Fodstad (1994) Expression of the Neuroectodermal Intermediate Filament Nestin in Human Melanomas more 3. Brychtova S, Fiuraskova M, Hlobilková A, Brychta T, Hirnak J. Nestin expression in cutaneous melanomas and melanocytic nevi. J Cutan Pathol. 2007 May;34(5):370-5. Anna Flammiger (2009) Expression von Nestin und Osteopontin in Melanomzelllinien und die Rolle der Transkriptionsfaktoren BRN2, SOX9 und SOX10 Dissertation Medizinischen Fakultät der Universität München (S. 1-95)
-
Tips
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. The preservative sodium azide has been added in very small quantities. Nevertheless, it may be harmful to health and the reagent should be used only by trained personnel. For waste disposal, please refer to national regulations.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus