GOAT IgG Fab fragment Biotin conjugated Protein
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Catalog number
OPRA03750
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Price
Please ask
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Size
one vial
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Storage
For short term storage GOAT IgG Fab fragment Biotin conjugated Protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.
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Tips
Due to handling and transportation, small amounts of the GOAT IgG Fab fragment Biotin conjugated Protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short name
GOAT IgG Fab conjugated Protein
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Technique
Goat, Fab, IgG, antibody Conjugates, fabs, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, Fab fragment, Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. IgG
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Label
Biotin
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Species
Goats
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Alternative name
caprine IgG Fab fragment Biotin conjugated peptide
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Alternative technique
caprine, seconairy, immunoglobulins
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