Leibovitz L-15 Medium w/L-Glutamine, HEPES, Tris, Low Sodium, Phosphate Free

• Catalog number
  PG059
• Price
  Please ask
• Size
  4X500ml

• Storage condition
  4°C
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Description
  There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Group
  media

• Gene target
  Leibovitz   L-15   w/L-Glutamine   Tris   Sodium   Phosphate   Free  
• Gene symbol
  PIRC66, PIRC71, PIRC75, PIRC67, PIRC72, PIRC68, PIRC62, PIRC70, PIRC76, PIRC77
• Short name
  Leibovitz L-15 Medium w/L-Glutamine, , Tris, Sodium, Phosphate Free
• Technique
  Medium, cell culture Media
• Alternative name
  Leibovitz L-15 Medium w/L-Glutamine, HEPES, Tris hydroxymethyl aminomethane, Low Sodium, Phosphate Free
• Alternative technique
  cellculture
• Virus
  hhv

Gene info

• Identity
  HGNC:37570
• Gene
  PIRC66
• Long gene name
  piwi-interacting RNA cluster 66
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313865
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37575
• Gene
  PIRC71
• Long gene name
  piwi-interacting RNA cluster 71
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313818
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37579
• Gene
  PIRC75
• Long gene name
  piwi-interacting RNA cluster 75
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313919
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37571
• Gene
  PIRC67
• Long gene name
  piwi-interacting RNA cluster 67
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313817
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37576
• Gene
  PIRC72
• Long gene name
  piwi-interacting RNA cluster 72
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313867
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37572
• Gene
  PIRC68
• Long gene name
  piwi-interacting RNA cluster 68
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313931
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37566
• Gene
  PIRC62
• Long gene name
  piwi-interacting RNA cluster 62
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313816
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37574
• Gene
  PIRC70
• Long gene name
  piwi-interacting RNA cluster 70
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313866
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37580
• Gene
  PIRC76
• Long gene name
  piwi-interacting RNA cluster 76
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313796
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

Gene info

• Identity
  HGNC:37581
• Gene
  PIRC77
• Long gene name
  piwi-interacting RNA cluster 77
• Locus
  15
• Discovery year
  2009-11-05
• Entrez gene record
  100313868
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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