DMEM High Glucose w/ stable Glutamine w/ 25mM Hepes w/o Sodium Pyruvate

  • Catalog number
    LM-D1113/500
  • Price
    Please ask
  • Size
    500 ml
  • Ordering information
    If you wish to order this product, please send us by email or by fax an official purchase order including your full shipping and billing information. Contact us if you need first a quotation. Delivery for USA and Europe next week if an order is received before Friday 2 pm.
  • Technical datasheet
    Please contact us to receive the technical datasheet or also the MSDS file
  • Contact
    Call us at +32 16 58 90 45 for more technical information about this product
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Gene target
    DMEM   Glucose   stable   Glutamine   25mM   w/o   Sodium   Pyruvate  
  • Short name
    DMEM Glucose w/ stable Glutamine w/ 25mM w/o Sodium Pyruvate
  • Alternative name
    DMEM High Glucose w/ stable Glutamine w/ 25mM Hepes w/o Sodium Pyruvate
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