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Available ordering format
Lyophilized
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Immunogen
Purified LHC complex from Porphyridium cruentum
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 200 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS09 407 | anti-Lhcb5 | CP26 (Lhcb5) homolog, ChlamydomonasAS09 408 | anti-Lhcbm5 | chlorophyll a-b binding protein of LHCIIAS06 117 | anti-Lhcb4 | CP29 (Lhcb4) homolog, Chlamydomonas
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Recommended dilutions for use
1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
20-25 kDa
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Verified reactivity
Aureococcus anophagefferens,Guillardia theta,Heterosigma akashiwo, Thalassiosira pseudonana,Porphyridium cruentum
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Possible reactivity
diatoms
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
strongly reactive to 7 Lhc1 light harvesting polypeptides of P. cruentum, reactivity to a few Lhc2 polypeptides of spinach is very low
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References
Tan et al. (1995).Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum. Photosyn. Research 45:1. Wolfe et al. (1994) Evidence for a common origin of chloroplasts with light-harvesting complexes of different pigmentation. Nature 367:566
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Scientific context
The light-harvesting system of higher plant is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.
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Notes
None
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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