Cultrex 3D Culture 10X Cell Harvesting Buffer

  • Catalog number
    3448-020-01
  • Price
    Please ask
  • Size
    100 ml
  • Assay performance
    Cultrexes are the best 3D matrixes for cell cultures known as matrigel or cultrex. Basal membrane extracts higher the differentiation capacity or from basal membrane over what can be achieved by 2D monolayer cultures from epithelial cells. You can use cultrex for high differentiation in a 3D matrix as Basement Membrane Extracts (BME), extracellular matrix from lumen epithelial or endothelial cells. BME, collagen I, collagen IV, laminin I, fibronectin and vitronectin are pathogen tested and suitable for 3d and pluripotent stem cell culture. Cultrex Basement Membrane and the PathClear Protocol is supplied in a liquid form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor at a concentration of 13 - 17 mg/ml from Murine Engelbreth-Holm-Swarm (EHS) tumor with Dulbecco’s Modified Eagle’s medium without phenol red and with 11 ug/ml gentamicin sulfate with cultrex BME for thin Layer Method (non-gelling).
  • More advice
    Cultrex 3D cell culture for cancer studies cell invasion and 3 dimensional cell expansion up to 2/3 conflunecy
  • Description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Trevigen. For cells, cell lines and tissues in culture till half confluency.
  • Test
    The gelatin protein mixture of Trevigen is called Cultrex and it is a matrigel like secreted supernatant of the EHS mouse sarcoma cell line. It is a 3D structure similar to Tissues and cells will to adhere to this basement membrane extract BME very well. Cultrexes are the best medium for 3D cell culture studies. They can be used as a metastasis model or an iPSC model (induced pluripotent stem cell). The cultrex medium is useful for cell migration studies using the Pathclear endotoxin free and sterile basement membrane extract. Enghelbreth Holm Swarm sarcoma cells are sold by Corning. A collagen, laminin, fibronectin and vitrobectin Cultrexes are available from Trevigen. Cultrex® Human has been tested for the viruses should be handled in a BSL2 ( biosafety level 2 ) environment.
  • Gene target
  • Short name
    Cultrex 3D Culture 10X Harvesting
  • Technique
    Cultrex, culture, cultrex
  • Alternative name
    Cultrex 3D Culture 10X cellular Harvesting solution
  • Alternative technique
    cultrexes
  • Tissue
    cell
MeSH Data
  • Name
  • Concept
    Scope note: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
  • Tree numbers
    • E05.481.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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