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Available ordering format
Liquid
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Immunogen
Polyhistidine (HHHHHH) tagged polypeptide
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
Clone AD1.1.10
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Purification
Protein A purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°CShelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Verified applications
Flow cytometry (FL), cell-based assays (CBA), microarrays, and microplate applications
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Connected products
AS15 2930 | 6x Histidine, HRP conjugated (mouse antibody)AS15 2935 | 6x Histidine, ALP Conjugated (mouse antibody)AS15 2934 | 6x Histidine, Biotin Conjugated (mouse antibody)AS15 2928 | 6x Histidine, conjugated to DyLight® 488 (mouse antibody)AS15 2929 | 6x Histidine, conjugated to DyLight® 550 (mouse antibody)AS15 2933 | 6x Histidine, conjugated to DyLight® 650 (mouse antibody)AS15 2932 | 6x Histidine, conjugated to Europium (mouse antibody)AS15 2931 | 6x Histidine, conjugated to SureLight® R-Phycoerythrin (mouse antibody)other tag antibodies
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Recommended dilutions for use
to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available, antibody released in August 2015
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Scientific context
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
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Notes
Antibody is provided in: 100 mM Sodium Phosphate pH 7.4, 100 mM NaCl, 50 mM Sucrose, 0.1% BSA and 2 mM Sodium Azide
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Conjugated
The polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
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Conjugation
histidine