Goat, M, FITC conjugated
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Catalog numberAS10 1005-200
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Price:
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Size200 ml (1.5 mg/ml)
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Available ordering formatLiquid
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Immunogento be determined
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Raised inGoat
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified goat IgG
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionStore at 2-8°C.Shelf life of this product is one year from date of receipt.
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Verified applicationsContact Gentaur support center
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Connected productsantibodies to human immunoglobulins fluorsecent dye conjugated
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Recommended dilutions for use1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC).
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Molecular weight expected аpparentsee datasheet
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Verified reactivityto be determined
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Possible reactivityto be determined
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No reactivityto be determined
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Supplementary informationContact Gentaur support center at [email protected]
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ReferencesRefer to PubMed
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Scientific contextGoat anti-human IgA + IgG + IgM is a secondary antibody, which binds to human IgA, IgG and IgM and is FITC (fluorescein-5-isothiocyanate) conjugated. Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgG + IgA + IgM.
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NotesThis antibody reacts with the heavy and light chains on human IgA, IgG and IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
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Protein numberRefer to NCBI
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TAIR numberRefer to NCBI
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PropertiesThis agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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Latin nameCapra aegagrus hircus
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Gene target
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Short nameGoat, M, FITC conjugated
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TechniqueFITC, Goat, antibody Conjugates, Fluorescein, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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LabelFITC
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SpeciesGoats
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Alternative namecaprine, M, fluorecein coupled
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Alternative techniquecaprine, fluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data