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Available ordering format
Liquid
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Immunogen
Purified rabbit IgG Fc.
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
2-8 °C. Shelf life is 1 year from date of receipt. Prepare working dilution prior use and then discard.
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Verified applications
ELISA (ELISA), Flow cytometry (FC), Immunofluorescence (IF), Immunolocalization (IL)
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Connected products
other FITC conjugated antibodies to rabbit IgG
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Recommended dilutions for use
The optimal working dilution should be determined by the investigator.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Rabbit IgG Fc.
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in October 2016.
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Scientific context
Goat anti-rabbit IgG Fc, FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Antibodies are adsorbed against bovine, horse and human serum proteins and affinity purified using solid phase rabbit IgG Fc.
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on rabbit IgG Based on IEP, no reactivity is observed to: - non-immunoglobulin rabbit serum proteins - light chains on all rabbit immunoglobulins - Rabbit IgG F(ab)'2 fragment - serum proteins from bovine, horse or human
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
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About
Serum should be stored at +5°C
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Gene target
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Short name
Goat, Fc, FITC conjugated, min. cross-reactivity , serum proteins
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Technique
serum, FITC, Goat, FC, antibody Conjugates, Fluorescein, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
FITC
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Species
Human, Bos Taurus genes are available form the Bovine Genome database. Goats, Horses, Humans
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Alternative name
caprine, fragment c, fluorecein coupled, min. cross-This (antibody to-) recognizes to bovine, horse and H. sapiens blood serum proteins
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Alternative technique
sera, caprine, fluorescine
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Tissue
serum
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