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Available ordering format
Lyophilized
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Immunogen
Purified Hamster IgG, whole molecule
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified
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How to reconstitute
For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store freeze-dried powder at 2-8 °C.
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Verified applications
Flow cytometry (FACS), Immunomicroscopy (IM)
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Connected products
other anti-hamster secondary antibodies
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Recommended dilutions for use
1:20 - 1:2 000 (FACS and IM)
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Hamster IgG (H&L)
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
To be added when available, antibody released in April 2016.
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Scientific context
Rabbit anti-hamster IgG (H&L), HRP conjugated, is a secondary antibody conjugated to HRP, which binds to Hamster IgG (H&L) in immunological assays.
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Notes
Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Hamster IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on hamster IgG - light chains on all hamster immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin hamster serum immunoglobulins - human serum proteins .
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Latin name
Oryctolagus cuniculus
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Description
Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Group
Sera
