Goat, Fc, FITC conjugated
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Catalog number
AS10 977
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Price
Please ask
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Size
2 mg
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Available ordering format
Liquid
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Immunogen
to be determined
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified goat IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
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Verified applications
Contact Gentaur support center
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Connected products
other fluorescent dye conjugated antibodies to human IgG
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Recommended dilutions for use
1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and for flow cytometry.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
Refer to PubMed
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Scientific context
Goat anti-human IgG Fc is a FITC conjugated secondary antibody which binds to human IgG upsilon in immunological assays. FITC (fluorescein-5-isothiocyanate) has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgG.
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Notes
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins. Minimum cross-reactivity is observed to human IgG, F(ab)'2 fragment based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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Gene target
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Short name
Goat, Fc, FITC conjugated
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Technique
FITC, Goat, FC, antibody Conjugates, Fluorescein, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Label
FITC
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Species
Goats
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Alternative name
caprine, fragment c, fluorecein coupled
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Alternative technique
caprine, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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