GLN1 GLN2, GS1 GS2 glutamine synthetase global antibody

• Catalog number
  AS08 295
• Price
  Please ask
• Size
  50 µl

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 50 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB)
• Connected products
  AS08 295PRE | GLN1 GLN2 | GS1 glutamine synthetase, pre-immune serumAS08 296 GLN | GS2, chloroplastic form of glutamine synthetasecollection of antibodies to proteins involved in nitrogen metabolismplant and algal protein extraction buffersecondary antibodies
• Recommended dilutions for use
  1:10 000 (WB)
• Molecular weight expected аpparent
  39-40 kDa (GLN1,cytoplasmic form) , 44-45 kDa (GLN2, chloroplastic form)
• Verified reactivity
  Arabidopsis thaliana, Eragrostis tef, Medicago truncatula, Spinacia oleracea, Zea mays, moss: Physcomitrella patens, red alga Gracilaria gracilis
• Possible reactivity
  Brachypodium distachyon, Brassica napus, Camellia sinensis, Citrus clementina, Daphnia magna, Datisca glomerata, Emiliania huxleyi, Eucalyptus grandis, Gazania splendens, Genlisea aurea, Glycine max, Helianthus annuus, Hordeum vulgare, Oryza sativa, Panax quinquefolius, Phaseolus angularis, Phytophthora cinnamomi, Populus trichocarpa, Saccharum officinarum, Securigera parviflora, Solanum lycopersicum, Solanum tuberosum, Stevia rebaudiana, Theobroma cacao, Triticum aestivum, Zea mays, Vitis labruscaGLN1 dicots including: Brassica napus, Phaseolus vulgaris, monocots including: Hordeum vulgare, Oryza sativa, trees: Pinus sylvestris, Populus sp., Zosteria marina GLN2 dicots including: Brassica napus, Glycine max, Phaseolus vulgaris, monocots including: Triticum aestivum, Oryza sativaGLN3: Zea mays,GLN1 in algae: Chlamydomonas reinhardii
• No reactivity
  no confirmed exceptions from predicted reactivity known presently
• Supplementary information
  to be added when available
• References
  Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98-108. Podgorska et al. (2013). Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)+ ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity. Plant Cell Environ. April 10. Brouwer et al. (2011) The Impact of Light Intensity on Shade-Induced Leaf Senescence. Plant Cell Environ. Dec. 15 (ahead of print).Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
• Scientific context
  Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts
• Notes
  The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.
• Protein number
  Q56WN1 , Q8LCE1 , Q9LVI8 , Q9FMD9
• TAIR number
  AT5G37600
• Hyperlink
• Properties
  If you buy Antibodies supplied by agisera they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  GLN1   GLN2   GS1   GS2   glutamine   synthetase   global  
• Gene symbol
  LINC02154, PNPLA4, OTUD6B-AS1, RAB27A, MYO5A, PUDP
• Short name
  GLN1 GLN2, GS1 GS2 glutamine synthetase global antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Alternative name
  GLN1 GLN2, GS1 GS2 glutamine synthetase global (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:53015
• Gene
  LINC02154
• Long gene name
  long intergenic non-protein coding RNA 2154
• Synonyms
  • GS1-600G8.5
• Locus
  Xp22.2
• Discovery year
  2017-01-05
• Pubmed identfication
  • 28028307
• Classification
  • Long intergenic non-protein coding RNAs
• VEGA ID
  OTTHUMG00000021149

Gene info

• Identity
  HGNC:24887
• Gene
  PNPLA4
• Long gene name
  patatin like phospholipase domain containing 4
• Synonyms gene name
  • patatin-like phospholipase domain containing 4
• Synonyms
  • DXS1283E
  • GS2
  • iPLA2eta
• GenBank acession
  • U03886
• Locus
  Xp22.31
• Discovery year
  2004-09-06
• Entrez gene record
  8228
• Pubmed identfication
  • 7806223
  • 16799181
  • 19029121
• RefSeq identity
  • NM_004650
• Classification
  • Patatin like phospholipase domain containing
• VEGA ID
  OTTHUMG00000021103
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:50466
• Gene
  OTUD6B-AS1
• Long gene name
  OTUD6B antisense RNA 1 (head to head)
• Synonyms
  • GS1-251I9.4
• Locus
  8q21.3
• Discovery year
  2014-04-30
• Entrez gene record
  100506365
• Pubmed identfication
  • 31156645
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000164022

Gene info

• Identity
  HGNC:9766
• Gene
  RAB27A
• Long gene name
  RAB27A, member RAS oncogene family
• Synonyms
  • RAB27
  • RAM
  • GS2
  • HsT18676
• GenBank acession
  • U38654
• Locus
  15q21.3
• Discovery year
  1996-11-15
• Entrez gene record
  5873
• Pubmed identfication
  • 7592656
• RefSeq identity
  • NM_004580
  • NM_183236
• Classification
  • RAB, member RAS oncogene GTPases
• VEGA ID
  OTTHUMG00000131959
• Locus Specific Databases
  • RAB27Abase: Mutation registry for Griscelli syndrome, type 2 (GS2)
  • Mutations of the Small Nucleotide-binding Protein 27a Gene
  • LRG_96

Gene info

• Identity
  HGNC:7602
• Gene
  MYO5A
• Long gene name
  myosin VA
• Synonyms gene
  • MYH12
• Synonyms gene name
  • myosin VA (heavy polypeptide 12, myoxin)
  • myosin VA (heavy chain 12, myoxin)
• Synonyms
  • MYO5
  • GS1
  • MYR12
• Synonyms name
  • myosin, heavy polypeptide kinase
  • myosin heavy chain 12
  • myoxin
  • myosin V
• Locus
  15q21.2
• Discovery year
  1993-09-23
• Entrez gene record
  4644
• Pubmed identfication
  • 8188282
  • 8022818
• RefSeq identity
  • NM_000259
• Classification
  • Myosin heavy chains, class V
• VEGA ID
  OTTHUMG00000137383
• Locus Specific Databases
  • MYO5Abase: Mutation registry for Griscelli syndrome, type 1 (GS1)
  • LRG_86

Gene info

• Identity
  HGNC:16818
• Gene
  PUDP
• Long gene name
  pseudouridine 5'-phosphatase
• Synonyms gene
  • FAM16AX
  • HDHD1A
  • HDHD1
• Synonyms gene name
  • family with sequence similarity 16, member A, X-linked
  • haloacid dehalogenase-like hydrolase domain containing 1A
  • haloacid dehalogenase-like hydrolase domain containing 1
• Synonyms
  • DXF68S1E
  • GS1
• GenBank acession
  • M86934
• Locus
  Xp22.31
• Discovery year
  2003-02-11
• Entrez gene record
  8226
• Pubmed identfication
  • 1734713
  • 1284467
  • 20722631
• RefSeq identity
  • NM_012080
• Classification
  • HAD Asp-based non-protein phosphatases
  • Haloacid dehalogenase like hydrolase domain containing
• VEGA ID
  OTTHUMG00000021101
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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