Anti - Glutamine Synthetase (Biotin)

• Catalog number
  MBS612900
• Price
  Please ask
• Size
  0.1 mg

• Products_type
  Antibody
• Products_short_name
  [Glutamine Synthetase]
• Products_name_syn
  [Glutamine Synthetase (Biotin)]
• Other_names
  [glutamine synthetase; Glutamine synthetase; glutamine synthetase; OTTHUMP00000035524; OTTHUMP00000035525; glutamine synthase; glutamate decarboxylase; glutamate--ammonia ligase; proliferation-inducing protein 43; cell proliferation-inducing protein 59; glutamate-ammonia ligase; Glutamate decarboxylase (EC:4.1.1.15); Glutamate--ammonia ligase]
• Other_gene_names
  [GLUL; GLUL; GS; GLNS; PIG43; PIG59; GLNS; GS]
• Clonality
  Polyclonal
• Reactivity
  Goat
• Specificity
  Recognizes Glutamine Synthetase. Assay by IEP resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glutamine Synthetase.
• Purity
  Purified by delipidation, salt fractionation and ion exchange chromatography
• Form
  Highly Purified Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin. Biotin/Protein Ratio:
• Concentration
  ~10mg/ml
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  ELISA, Western Blot, Immunohistochemistry
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated

• Gene target
  Glutamine   Synthetase  
• Gene symbol
  ASNS, LGSN, QARS1
• Short name
  Anti - Glutamine Synthetase ( )
• Technique
  anti, antibody to
• Host
  Goat
• Isotype
  IgG
• Label
  Biotin
• Alternative name
  antibody to - Glutamine Synthetase (biotinilated)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:753
• Gene
  ASNS
• Long gene name
  asparagine synthetase (glutamine-hydrolyzing)
• Synonyms gene name
  • asparagine synthetase
• GenBank acession
  • M27396
• Locus
  7q21.3
• Discovery year
  2001-06-22
• Entrez gene record
  440
• Pubmed identfication
  • 7904551
• RefSeq identity
  • NM_001673
  • NM_183356
• VEGA ID
  OTTHUMG00000022892

Gene info

• Identity
  HGNC:21016
• Gene
  LGSN
• Long gene name
  lengsin, lens protein with glutamine synthetase domain
• Synonyms gene
  • GLULD1
• Synonyms gene name
  • glutamate-ammonia ligase (glutamine synthetase) domain containing 1
• Synonyms
  • LGS
• GenBank acession
  • AF242388
• Locus
  6q12
• Discovery year
  2003-05-02
• Entrez gene record
  51557
• Pubmed identfication
  • 12107412
• RefSeq identity
  • NM_016571
• VEGA ID
  OTTHUMG00000014946

Gene info

• Identity
  HGNC:9751
• Gene
  QARS1
• Long gene name
  glutaminyl-tRNA synthetase 1
• Synonyms gene
  • QARS
• Synonyms gene name
  • glutaminyl-tRNA synthetase
• Synonyms name
  • glutamine tRNA ligase
• GenBank acession
  • X76013
• Locus
  3p21.31
• Discovery year
  1994-12-13
• Entrez gene record
  5859
• Pubmed identfication
  • 8078941
  • 10393422
• RefSeq identity
  • NM_005051
• Classification
  • Aminoacyl tRNA synthetases, Class I
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000156774

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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