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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from known bacterial sequences of FtsZ including E.coli P0A9A6
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunofluorescence (IF)
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Connected products
AS07 217 | FtsZ | procaryotic cell division GTPase, cyanobacterial, rabbit antibodyAS09 413 | anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibody AS13 2651 | anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibodySecondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB), 1:100 - 1: 200 (IF)
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Molecular weight expected аpparent
40 | 42 kDa
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Verified reactivity
E coli DH5a, Shigella flexneri
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Possible reactivity
Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038
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No reactivity
B. subtilis, Listera sp., Pseudomonas aeruginosa, cyanobacteria
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Supplementary information
to be added when available
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References
Pende et al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803. Söderström et al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
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Scientific context
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.
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Notes
None
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Protein number
P0A9A6
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TAIR number
Refer to NCBI
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Description
For cells, cell lines and tissues in culture till half confluency.
