DYKDDDDK, FLAG®), ALP conjugated
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Catalog number
AS15 2952
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Price
Please ask
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Size
100 µg
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Available ordering format
Lyophilized
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Immunogen
DYKDDDDK (Sigma FLAG®)
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Raised in
Mouse
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Clonality
Monoclonal
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Clone
Clone M2
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Purification
Protein A purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C.Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Verified applications
Western blot (WB)
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Connected products
AS15 2950 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 488 conjugated (mouse antibody)AS15 2951 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 550 conjugated (mouse antibody)AS15 2947 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 650 conjugated (mouse antibody)AS15 2948 | anti-DYKDDDDK (binds to Sigma FLAG®), Europium conjugated (mouse antibody)AS15 2949 | anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated (mouse antibody)AS15 3036 | anti-DYKDDDDK (binds to Sigma FLAG®), (mouse antibody) AS15 3037 | anti-DYKDDDDK (binds to Sigma FLAG®), (rabbit antibody)other tag antibodies
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Recommended dilutions for use
to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available, antibody released in September 2015
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Scientific context
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK.
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Notes
Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH7.4)
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Gene target
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Gene symbol
PDLIM3, ASRGL1, ATRNL1, SLPI, CCL27
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Short name
DYKDDDDK, ®), ALP conjugated
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Technique
antibody Conjugates
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Label
Flag
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Alternative name
DYKDDDDK, FLAG®), ALP coupled
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Gene info
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Identity
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Gene
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Long gene name
PDZ and LIM domain 3
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Synonyms
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GenBank acession
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Locus
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Discovery year
2004-02-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PDZ domain containing
- LIM domain containing
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
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Identity
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Gene
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Long gene name
attractin like 1
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
2004-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C-type lectin domain containing
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VEGA ID
Gene info
Gene info
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