Donkey, L), Biotin conjugated

  • Catalog number
    AS10 1305
  • Price
    Please ask
  • Size
    2 mg
  • Available ordering format
    Liquid
  • Immunogen
    to be determined
  • Raised in
    Donkey
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified IgG
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    Store at 2-8°C.Shelf life of this product is one year from date of receipt.
  • Verified applications
    Contact Gentaur support center
  • Connected products
    other biotinylated secondary antibodies to goat IgG
  • Recommended dilutions for use
    Needs to be determined by end user for all immunoassay applications.
  • Molecular weight expected аpparent
    see datasheet
  • Verified reactivity
    to be determined
  • Possible reactivity
    to be determined
  • No reactivity
    to be determined
  • Supplementary information
    Contact Gentaur support center at [email protected]
  • References
    Refer to PubMed
  • Scientific context
    Donkey anti-goat IgG (H&L), biotin conjugated is a secondary antibody which binds to goat IgG in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
  • Notes
    This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Latin name
    Equus africanus asinus
  • Gene target
  • Short name
    , L), conjugated
  • Technique
    antibody Conjugates
  • Host
    Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
  • Label
    Biotin
  • Species
    Donkeys
  • Alternative name
    Donkey, L), biotinilated coupled
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