DONKEY IgG F(ab')2 fragment Biotin conjugated Protein

Storage

For short term storage DONKEY IgG F(ab')2 fragment Biotin conjugated Protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.
Tips

Due to handling and transportation, small amounts of the DONKEY IgG F(ab')2 fragment Biotin conjugated Protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Latin name

Equus africanus asinus
Group

f(ab')2s
About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Gene target

conjugated Protein
Short name

IgG F(ab')2 conjugated Protein
Technique

F(ab')2 fragment, F(ab')2, IgG, antibody Conjugates, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. f(ab')2 fragments, IgGs
Host

Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
Isotype

IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, Mouse IgG1 Fab and F(ab')2 Preparation Kit , Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common. IgG
Label

Biotin
Species

Donkeys
Alternative name

DONKEY IgG F(ab')2 fragment Biotin conjugated peptide
Alternative technique

secondairy, immunoglobulins