Donkey, IgG or serum proteins

• Catalog number
  AS16 3508
• Price
  Please ask
• Size
  2.0 mg

• Available ordering format
  Liquid
• Immunogen
  Purified Rabbit IgG, whole molecule
• Raised in
  Donkey
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified
• How to reconstitute
  See included datasheet or contact our support service
• Storage condition
  Store at 2-8 °C.
• Verified applications
  Flow cytometry (FACS), Immunomicroscopy (IM)
• Connected products
  other anti-rabbit secondary antibodies
• Recommended dilutions for use
  1:20 - 1:2 000 (FACS and IM)
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  Rabbit IgG (H&L)
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  To be added when available, antibody released in April 2016.
• Scientific context
  Donkey anti-Rabbit IgG (H&L), Biotin conjugated, min. cross-reactivity to mouse IgG or serum proteins is a secondary antibody conjugated to biotin, which binds to rabbit IgG in immunological assays.
• Notes
  Antibody purity is > 95% based on SDS-PAGE.Affinity purified using solid phase Rabbit IgG .Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.Based on IEP, this antibody reacts with: - heavy (gamma) chains on rabbit IgG - light chains on all rabbit immunoglobulins . Based on IEP, no reactivity is observed to: - non-immunoglobulin rabbit serum proteins - mouse IgG or serum proteins .
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Latin name
  Equus africanus asinus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
• Description
  Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
• Group
  Sera

• Gene target
  proteins  
• Gene symbol
  PGAP1, PGAP3, MPPE1
• Short name
  , IgG serum proteins
• Technique
  serum, IgG, IgGs
• Host
  Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
• Isotype
  IgG, IgG
• Species
  Donkeys
• Alternative name
  Donkey, Immunoglobulin G and blood serum proteins
• Alternative technique
  sera, immunoglobulins
• Tissue
  serum

Gene info

• Identity
  HGNC:25712
• Gene
  PGAP1
• Long gene name
  post-GPI attachment to proteins inositol deacylase 1
• Synonyms gene name
  • post-GPI attachment to proteins 1
• Synonyms
  • FLJ12377
  • Bst1
  • SPG67
• Synonyms name
  • GPI inositol-deacylase
• Locus
  2q33.1
• Discovery year
  2008-02-26
• Entrez gene record
  80055
• Pubmed identfication
  • 14734546
  • 17711852
  • 24482476
• RefSeq identity
  • NM_024989
• VEGA ID
  OTTHUMG00000132743

Gene info

• Identity
  HGNC:23719
• Gene
  PGAP3
• Long gene name
  post-GPI attachment to proteins phospholipase 3
• Synonyms gene
  • PERLD1
• Synonyms gene name
  • per1-like domain containing 1
  • post-GPI attachment to proteins 3
• Synonyms
  • MGC9753
  • CAB2
  • PP1498
  • PER1
• Synonyms name
  • post-GPI attachment to proteins 3
• GenBank acession
  • AB088396
• Locus
  17q12
• Discovery year
  2004-04-30
• Entrez gene record
  93210
• Pubmed identfication
  • 15010812
  • 17021251
  • 17314402
• RefSeq identity
  • NM_033419
• VEGA ID
  OTTHUMG00000179110

Gene info

• Identity
  HGNC:15988
• Gene
  MPPE1
• Long gene name
  metallophosphoesterase 1
• Synonyms
  • PGAP5
  • Cdc1
• Synonyms name
  • post-GPI attachment to proteins 5
• GenBank acession
  • BC002877
• Locus
  18p11.21
• Discovery year
  2003-10-17
• Entrez gene record
  65258
• Pubmed identfication
  • 11978971
  • 19837036
• RefSeq identity
  • NM_023075
• VEGA ID
  OTTHUMG00000131661

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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