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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Donkey anti-Rabbit IgG (H&L) - Affinity Pure]
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Reactivity
Rabbit. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin rabbit serum proteins mouse IgG or serum proteins
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Rabbit IgG.
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Form
Lyophilized
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Concentration
1.0 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
Flow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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Test
A high affinity purification column was use to purify anti-Rabbit IgG (H L) - Pure, TRITC Conjugate, min x w/mouse IgG serum proteins by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure anti-Rabbit IgG (H L) - Pure, TRITC Conjugate, min x w/mouse IgG serum proteins supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Latin name
Equus africanus asinus, Mus musculus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Group
Sera
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Gene target
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Short name
anti-Rabbit IgG (H L) - Pure, TRITC Conjugate, min x w/mouse IgG serum proteins
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Technique
affinity, Rabbit, anti-, Mouse, serum, anti, Pure, IgG, antibody to, antibodies, IgGs, mouses, pure products
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Host
Donkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
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Isotype
IgG, Heavy & Light chains, IgG
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Label
tritc
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Species
Mouse, Donkeys, Mouses
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Alternative name
Donkey Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) - protein Pure, TRITC Conjugate, min x w/mouse Immunoglobulin G and blood serum proteins
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Alternative technique
rabbit-anti, sera, murine, antibodies, purified, immunoglobulins
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Tissue
serum
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