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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum in PBS pH 7.4.
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodyAS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated) AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP | Anti-AGO1 | argonaute 1 (40 µg, HRP-conjugated) AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P | AGO1 | argonaute 1 | Blocking peptideAS09 617 | Anti-AGO4 argonaute 4, rabbit antibodyAS10 671 | Anti-AGO5 | argonaute 5, rabbit antibodyAS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodyAS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodyAS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 250 - 1: 500 (WB)
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Molecular weight expected аpparent
113 kDa
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Verified reactivity
Arabidopsis thaliana
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Possible reactivity
Capsella rubella, Solanum tuberosum
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No reactivity
Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays
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Supplementary information
AGO2 protein is strongly induced by stress. AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Antibody incubation should be done over night in 4°C. Use of material with enriched AGO2 levels is recommened.
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References
To be added when available, antibody released in April 2014.
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Scientific context
AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). AGO2 is probably involved in antiviral RNA silencing.
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Notes
TCA acetone total protein precipitation method
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Protein number
Q9SHF3
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TAIR number
AT1G31280
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