Recombinant purified FLAG tag-Protein (~9 kda) control for ELISA

• Catalog number
  FLAG15-R
• Price
  Please ask
• Size
  100 ug

• Stock availability
  Available
• Category
  Recombinant Protein
• Antibody type
  N/A
• Antibody host
  N/A
• Antibody conjugate
  His-Tag?
• Technical datasheet
  Contact Gentaur to request the datasheet or ask our specialists for more information.
• Notes
  The Recombinant purified FLAG tag-Protein (~9 kda) control for ELISA is manufactured for Research Use Only or for diagnostics purposes.
• Other related products
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples. The kilo Daltons subunit weight of Recombinant purified tag-Protein (~9 ) control for ELISA compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
• Conjugation
  Flag
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  tag-Protein   for  
• Short name
  Recombinant purified tag-Protein (~9 ) control for ELISA
• Technique
  Recombinant, purified, Control, ELISA, controls, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. purifications, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. adi advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  antigen assay ELISA kit 1 Kit (96 Wells)
• Label
  Flag
• Species
  N/A
• Alternative name
  Rec. enriched FLAG detection labelled-Protein (~9 kiloDalton) reference to measure Enzyme-linked immunosorbent assay
• Alternative technique
  rec, pure, controls, elisas
• Tissue
  control

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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